Songs without Words

No way.

No way.

No freaking way.

So today, on experiment 7.12, as in, twelfth try at this experiment, because it has hardly changed since IAP.  I prepare the antibody dilution.  And then I stop.  Something in my head clicks.  More like, a loud voice starts booming:

WHAT THE HELL ARE YOU DOING?!

The months of troubleshooting … messing around with detergent concentration, ordering new antibodies, aliquoting them to prevent freeze-thaw, flash freezing them with liquid nitrogen, varying the incubation time, changing cell types … and yet, it’s quite apparent, indeed, GLARINGLY apparent

Common knowledge for this protocol: I’m using TBS and PBS saline solutions in this experiment.  Why?  Because water ruptures the cells, obviously.

SO WHY AM I DILUTING THE ANTIBODIES IN WATER INSTEAD OF BLOCKING SOLUTION?!

I still don’t want to believe this.  It’s like hunting for dinosaurs by hopping on the back of a T. Rex.

Somehow, I think that it’s gonna take a LOT of DNA animations and thank you cards to get me out of the dungeon my professor will lock me in once she finds out about this.

In the meantime, I have to start over again.  7.13.  Lucky 13, right?